CK20 and lymph node involvement predict adverse outcome of malignant intraductal papillary neoplasm of the bile duct

Jie Shi¹, Xueshuai Wan², Yuan Xie³, Jianzhen Lin², Junyu Long², Weiyu Xu², Zhiyong Liang¹, Xinting Sang² and Haitao Zhao<sup>2

1</sup>Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, ²Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing and ³Department of Hepatobiliary Surgery II, Zhujiang Hospital, Southern Medical University, Guangzhou, PR China
Jie Shi and Xueshuai Wan contributed equally to this work

Offprint requests to: Xinting Sang, MD, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC), 1 Shuaifuyuan, Wangfujing, Beijing 100730, China. e-mail: sangxt@pumch.cn. or Haitao Zhao, MD, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC), 1 Shuaifuyuan, Wangfujing, Beijing 100730, China. e-mail: zhaoht@pumch.cn

Summary. Objectives. To identify prognostic factors of malignant intraductal papillary neoplasm of the bile duct (m-IPNB). Materials and Methods. We included 38 consecutive cases which underwent surgical resection and diagnosed as IPNB with malignant component from January 2003 to January 2017. Clinicopathological variables were collected to conduct survival analysis and identify prognostic factors. Results. The median overall survival (OS) of m-IPNB was 76.0 months, with 1-, 3-, and 5-year survival rates of 97.2%, 73.5%, and 59.8%, respectively. The median recurrence-free survival (RFS) was 48.0 months with 1-, 3-, and 5-year RFS rate was 83.2%, 59.8%, and 44.6%, respectively. Univariate analysis showed that elevation of carcinoembrionic antigen (CEA), lymph node involvement, resection margin status, degree of periductal invasion, and positive expression of CK20 were associated with both OS and RFS of m-IPNB. After multivariate Cox models analysis, lymph node involvement and positive expression of CK20 were identified as independent prognostic factors for OS, while lymph node involvement and resection margin status were independent prognostic factors for RFS. The median OS of patients with m-IPNB involving lymphatic metastases and positive expression of CK20 was 27.0±8.8 months and 51.0±12.4 months, respectively. The median RFS of cases with lymph node involvement and R1 resection was 10.0±3.3 months and 25.0±6.9 months, respectively. However, there was no significant difference in OS or RFS between cases of pancreaticobiliary and intestinal subtype. Conclusions. Lymph node involvement and positive expression of CK20 are independent prognostic factors for shorter OS of m-IPNB, while patients with lymph node involvement and positive resection margin are at higher risk of tumor recurrence. Histol Histopathol 35, 449-456 (2020)

Key words: CK20, Lymph node, Papillary neoplasm, Bile duct, Prognostic factors

DOI: 10.14670/HH-18-179