Long noncoding RNA small nucleolar RNA host gene 12 promotes papillary thyroid carcinoma cell growth and invasion by targeting miR16-5p
Xiaocheng Feng1, Xuehong Dong1, Dingting Wu2, Hanxin Zhao1, Changqin Xu3 and Hong Li1
1Department of Endocrinology and Metabolism, Zhejiang University Affiliated Sir Run Shaw Hospital, School of Medicine, Hangzhou, 2Department of Nutrition Division, Zhejiang University Affiliated Fourth Hospital, School of Medicine, Yiwu and 3Department of Pneumology, Hangzhou Normal University Affiliated Hospital, Hangzhou, PR China
Offprint requests to: Xiaocheng Feng, MD, Department of Endocrinology and Metabolism, Zhejiang University Affiliated Sir Run Shaw Hospital, School of Medicine, No 368 Xia Sha Road Hangzhou Economic and Technological Development Zone, Hangzhou, 310016, Zhejiang, China. e-mail: fengxc@zju.edu.cn
Summary. Emerging evidence has shown that long noncoding RNA (lncRNA) plays an important role in various types of malignant cancer. Small nucleolar RNA host gene 12 (SNHG12) was found to be upregulated and to act as an oncogene in several cancers. However, the function and regulatory mechanism of SNHG12 remain unclear in papillary thyroid carcinoma (PTC). In this study, SNHG12 was found to be increased in PTC tissues and cell lines using quantitative real-time PCR. Knockdown of SNHG12 significantly inhibited PTC cell proliferation, migration and invasion and induced apoptosis in vitro. Mechanistic investigations revealed that SNHG12 functions as a competing endogenous RNA (ceRNA) to sponge miR-16-5p, which was downregulated in PTC tissues. In addition, rescue assays further confirmed that SNHG12 contributed to the progression of PTC through regulating miR-16-5p expression. These results indicated that SNHG12 might contribute to tumor progression in PTC by acting as a ceRNA to sponge miR-16-5p. Histol Histopathol 35, 217-224 (2020)
Key words: SNHG12, PTC, miR-16-5p
DOI: 10.14670/HH-18-155