HISTOLOGY AND HISTOPATHOLOGY

From Cell Biology to Tissue Engineering

 

Protective effect of alpha-mangostin on thioacetamide-induced liver fibrosis in rats as revealed by morpho-functional analysis

Siripa Rodniem, Vilailak Tiyao, Cheng Nilbu-nga, Raksawan Poonkhum, Sirinun Pongmayteegul and Wisuit Pradidarcheep

Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand

Offprint requests to: Wisuit Pradidarcheep, PhD., Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23 Rd, Bangkok 10110, Thailand. e-mail: wisuit@g.swu.ac.th, pthongp@gmail.com


Summary. Liver fibrosis is an excessive accumulation of scar tissue resulting from inflammation and cell death. Thioacetamide (TAA) is a well-known hepatotoxin that induces liver fibrosis. A marker of injured hepatocytes is transforming growth factor-beta 1 (TGF-β1), while alpha-smooth muscle actin (α-SMA) and tissue inhibitor of metalloproteinase 1 (TIMP-1) are markers of activated hepatic stellate cells. Alpha-mangostin, a major xanthone derivative from the mangosteen pericarp, has been shown to have anti-oxidant and anti-inflammatory activities. The objective of this study was to determine whether alpha-mangostin has a protective effect on TAA-induced liver fibrosis in rats. The rats were treated by intraperitoneal injection of compounds for eight weeks. For the control group a mixture of dimethyl sulfoxide and phosphate buffered saline was administered. Two hundred mg/kg BW of TAA was administered three times weekly. Alpha-mangostin was administered at 5 mg/kg BW and silymarin at 100 mg/kg BW, both twice weekly. TAA induced histologically recognizable liver damage and fibrosis, as anticipated. Furthermore, it increased immunohistochemically detectable TGF-β1, α-SMA, and TIMP-1. Co-administration of alpha-mangostin or silymarin with TAA prevented or ameliorated the effects of TAA administration alone. The anti-fibrotic effect of alpha-mangostin was stronger than that of silymarin. Histol Histopathol 34, 419-430 (2019)

Key words: Liver fibrosis, Thioacetamide, Alpha-mangostin, Herb

DOI: 10.14670/HH-18-052