From Cell Biology to Tissue Engineering


Comparison of histological characteristics and expression of CD3 and CD79a among the hemal nodes, lymph nodes and spleens of yaks (Bos grunniens)

Yufeng Huang1, Yan Cui1,2, Sijiu Yu2, Junfeng He1, Yanyu He1, Qian Zhang1, Penggang Liu1, Yanhuan Pu1, Juan Sun1 and Xinhua Kang1

1Laboratory of Animal Anatomy and Tissue Embryology, Department of Basic Veterinary Medicine and 2Gansu Province Livestock Embryo Engineering Research Center, Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China

Offprint requests to: Prof. Yan Cui, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China. e-mail: cuiyan369@sina.com

Summary. This experiment compared the histological characteristics and distribution of CD3 and CD79a among yak lymph nodes, hemal nodes and spleen. The three organs from ten clinically healthy newborn and adult yaks were studied using histology and immunohistochemistry. The yak hemal nodes, which consisted of blood sinuses, lymphoid follicles, diffuse lymphoid tissue and lymphoid cords, appeared to share the histological characteristics of the spleen and lymph nodes: the lymphoid follicles of the hemal nodes were much like those of the lymph nodes, which were not surrounded by the central artery and periarteriolar lymphoid sheath. The lymphoid cords of the hemal node, which contained many erythrocytes, were much like the splenic cords. The sinuses of the hemal nodes had a similar structure to the lymph sinuses of the lymph nodes but were engorged with erythrocytes rather than lymph as in the lymph nodes. Interestingly, the splenic sinuses of yak were of two different types: the sinuses with obvious endothelial cells or those consisting of reticular cells. The CD3+ cells were mainly located in the paracortex area and medulla of the lymph nodes, the diffuse lymphoid tissues of the hemal nodes, and the periarteriolar lymphoid sheaths and red pulp of the spleen. Most CD79a+ cells were mainly detected in the lymphoid follicles of all examined lymphoid organs. The results suggested that although the three organs had specific characteristics, in some respects, they had similar organizational structural characteristics and immune functions. These may be useful to better understand the relationship between the morphology and function of these organs and provide useful references for normal yak lymphoid organs. Histol Histopathol 34, 241-256 (2019)

Key words: Yak, Hemal node, Sinuses, Lymphoid follicles

DOI: 10.14670/HH-18-030