Preliminary analysis of the association of TRPV1 to the formation of Marfan syndrome aneurysms

María Elena Soto¹, Elizabeth Soria-Castro², Verónica Guarner-Lans³, Andrés Martínez Guzmán⁴, Cesar Amilcar Morales Marín⁴, Karla Susana Martínez Zavala¹ and Israel Pérez-Torres<sup>2

1</sup>Immunology Department, ²Pathology Department, ³Physiology Department and ⁴Cardiovascular Surgery Department, National Institute of Cardiology "Ignacio Chávez", Tlalpan, México City, México

Offprint requests to: Israel Pérez-Torres, Pathology Department, National Institute of Cardiology "Ignacio Chávez", Juan Badiano 1, Sección XVI, Tlapan, 14080 México City, México. e-mail: pertorisr@yahoo.com.mx

Summary. Marfan syndrome (MS) is an autosomal dominant disorder of connective tissue that is caused by mutations in the fibrillin-1 (FBN-1) gene that cause degeneration of the artery. It is accompanied by endothelial dysfunction. The potential transient receptor of the vanilloid subfamily 1 (TRPV1) ion channel plays an important role in endothelial vascular functioning. Here we determine the association of the presence TRPV1 in aortic aneurysm with dilation and dissection of the artery in MS patients. Histological sections of aortic aneurysm tissue obtained by the surgical procedure of Bentall and De Bono or David, were processed by immunohistochemistry with antibodies against ICAM, VCAM, iNOS, eNOS, TRPV1 and TNF-α and the immunolabelling area was determined. We also measured the NO₃^-/NO₂^- ratio in the aortic tissue. C-reactive protein and HDL in plasma were quantified. A significant increase in iNOS, TRPV1, VCAM (p<=0.05), NO₃^-/NO₂^- ratio (p=0.002) and a significant decrease in eNOS (p=0.04) and HDL in plasma (p=0.02) in the MS vs. the C group were found. Conclusion: TRPV1 is over-expressed in aortic tissue from MS patients and can be associated with increases in iNOS, VCAM and a decrease in eNOS. These changes might contribute to the progression and rupture of the thoracic aneurysm. Histol Histopathol 34, 1329-1343 (2019)

Key words: Marfan syndrome, TRPV1, eNOS, iNOS, Thoracic aneurysm

DOI: 10.14670/HH-18-127