HISTOLOGY AND HISTOPATHOLOGY

From Cell Biology to Tissue Engineering

 

Model of human recurrent respiratory papilloma on chicken embryo chorioallantoic membrane for tumor angiogenesis research

Virgilijus Uloza1, Alina Kuzminienė1, Jolita Palubinskienė2, Ingrida Balnytė2, Ingrida Ulozienė1 and Angelija Valančiūtė2

1Department of Otorhinolaryngology and 2Department of Histology and Embryology, Lithuanian University of Health Sciences, Kaunas, Lithuania
All authors contributed equally

Offprint requests to: Alina Kuzminienė, Department of Otorhino-laryngology, Lithuanian Uniersity of Health Sciences, Kaunas, Lithuania. e-mail: alinakuzminiene@gmail.com


Summary. We aimed to develop a chick embryo chorioallantoic membrane (CAM) model of recurrent respiratory papilloma (RPP) and to evaluate its morphological and morphometric characteristics, together with angiogenic features. Fresh RRP tissue samples obtained from 13 patients were implanted in 174 chick embryo CAMs. Morphological, morphometric, and angiogenic changes in the CAM and chorionic epithelium were evaluated up until 7 days after the implantation. Immunohisto-chemical analysis (34βE12, Ki-67, MMP-9, PCNA, and Sambucus nigra staining) was performed to detect cytokeratins and endothelial cells and to evaluate proliferative capacity of the RRP before and after implantation on the CAM. The implanted RRP tissue samples survived on CAM in 73% of cases while retaining their essential morphologic characteristics and proliferative capacity of the original tumor. Implants induced thickening of both the CAM (241-560%, p=0.001) and the chorionic epithelium (107-151%, p=0.001), while the number of blood vessels (37-85%, p=0.001) in the CAM increased. The results of the present study confirmed that chick embryo CAM is a relevant host for serving as a medium for RRP fresh tissue implantation. The CAM assay demonstrated the specific RRP tumor growth pattern after implantation and provided the first morphological and morphometric characterization of the RRP CAM model that opens new horizons in studying this disease. Histol Histopathol 32, 699-710 (2017)

Key words: Recurrent respiraory papilloma, Choriallantoic membrane, Angiogenesis

DOI: 10.14670/HH-11-831