HISTOLOGY AND HISTOPATHOLOGY

From Cell Biology to Tissue Engineering

 

The expression of MMP-14 and microRNA-410 in FFPE tissues of human endometrial adenocarcinoma

Beata Rak1,2, Filip Garbicz1, Wiktor Paskal1, Kacper Pełka1, Janina Maja Marczewska3, Dominika Wołosz3 and Paweł Włodarski1

1Department of Histology and Embryology, Center for Biostructure Research, Medical University of Warsaw, 2Postgraduate School of Molecular Medicine and 3Department of Pathology, Center for Biostructure Research, Medical University of Warsaw, Warsaw, Poland

Offprint requests to: Paweł Włodarski, Department of Histology and Embryology, Center for Biostructure Research, Medical University of Warsaw, ul. Chałubińskiego 5, 02-004 Warsaw, Poland. e-mail: pawel.wlodarski@wum.edu.pl


Summary. Endometrial cancer (EC) is the most common gynecological malignancy in Europe and North America. It is classified into two types exhibiting different characteristics and prognosis. Type I is an estrogen-dependent tumor, histologically classified as low grade and low stage, usually with an excellent prognosis. Type II EC is unrelated to estrogen stimulation and is characterized by a poor prognosis. MicroRNAs (miRNAs, miRs) are small non-coding RNA polynucleotides that regulate gene expression post-transcriptionally. Various dysregulations in microRNA expression are often considered to have an impact on the diagnosis, prognosis and overall survival in patients diagnosed with different types of cancers. Recent data suggest that microRNAs play an important role in the pathogenesis of EC. The aim of the study was to evaluate the involvement of matrix metaloprotease 14 (MMP-14) and microRNA-410 in formation of the EC tumor. To this end expression of MMP-14 and microRNA-410 was assessed within the cancer, transient and healthy zones in the histological sections of tumours using immunohisto-chemical staining and laser capture microdissection (LCM) followed by a quantitative real-time PCR. The results revealed significantly higher expression of MMP-14 in the cancer tissue zone in comparison to the healthy tissue zone, as well as a lower expression of microRNA-410 in the cancer zone compared with the healthy zone. This reverse correlation may suggest a regulatory role of miRNA-410 in modulating levels of MMP-14 in EC. This is the first report on such regulation in human endometrial cancer. Histol Histopathol 31, 911-920 (2016)

Key words: Endometrial cancer, Micro-RNA-410, MMP-14

DOI: 10.14670/HH-11-728