From Cell Biology to Tissue Engineering



Role of skeletal muscle in motor neuron development

Mark Baguma-Nibasheka1, Anna Fracassi2, Willard J. Costain3, Sandra Moreno2 and Boris Kablar1

1Department of Medical Neuroscience, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada, 2Department of Science, LIME, University “Roma Tre”, Rome, Italy and 3Glycosyltransferases and Neuroglycomics, Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada

Offprint requests to: Dr. Boris Kablar, Department of Medical Neuroscience, Faculty of Medicine, Dalhousie University, 5850 College Street, PO Box 15000, Halifax, NS, B3H 4R2 Canada. e-mail: bkablar@dal.ca

Summary. The current paper is a continuation of our work most recently described in Kablar, 2011. Here, we show lists of up- and down-regulated genes obtained by a cDNA microarray analysis that compared developing mouse MyoD-/- limb musculature (MyoD-dependent, innervated by Lateral Motor Column motor neurons) and Myf5-/- back (epaxial) musculature (Myf5-dependent, innervated by Medial Motor Column motor neurons) to the control and to each other, at embryonic day 13.5 which coincides with the robust programmed cell death of motor neurons and the inability of myogenesis to undergo its normal progression in the absence of Myf5 and MyoD that at this embryonic day cannot substitute for each other. We wanted to see if/how the myogenic program couples with the neurotrophic one, and also to separate Lateral from Medial column trophic requirements, potentially relevant to Motor Neuron Diseases with the predilection for the Lateral column. Several follow-up steps revealed that Kif5c, Stxbp1 and Polb, differentially expressed in the MyoD-/- limb muscle, and Ppargc1a, Glrb and Hoxd10, differentially expressed in the Myf5-/- back muscle, are actually regulators of motor neuron numbers. We propose a series of follow-up experiments and various ways to consider our current data. Histol Histopathol 31, 699-719 (2016)

Key words: MyoD, Myf5, Myogenesis, Neurotrophins, Motor neuron, Microarray

DOI: 10.14670/HH-11-742