HISTOLOGY AND HISTOPATHOLOGY

From Cell Biology to Tissue Engineering

 

Diagnostic value of matrix metalloproteinase 9 and tissue inhibitor of matrix metalloproteinases 1 in cholesteatoma

Ewa Olszewska1, Marlena Matulka1, Barbara Mroczko2, Anna Pryczynicz3, Andrzej Kemona3, Maciej Szmitkowski4, Jozef Mierzwinski5 and Tymoteusz Pietrewicz6

1Department of Otolaryngology, Medical University of Bialystok, 2Department of Neurodegeneration Diagnostics, Medical University of Bialystok, 3Department of General Pathomorphology, Medical University of Bialystok, 4Department of Biochemical Diagnostics, Medical University of Bialystok, 5Department of Pediatric Otolaryngology, Bydgoszcz and 6Post Graduating Training, Teaching Hospital of Bialystok, Poland

Offprint requests to: Ewa Olszewska, Department of Otolaryngology, Medical University of Bialystok, Ul. Sklodowskiej 24 A, 15-276 Bialystok, Poland. e-mail: Ewa.Olszewska@umwb.edu.pl


Summary. Objectives: Matrix metalloproteinase 9 (MMP-9), able to degrade type IV collagen, plays a key role in inflammatory cell migration as well as in the destructive behaviour of cholesteatoma. The aim of our study was to compare the expression of MMP-9 and TIMP-1 in cholesteatoma tissue and in the concentrations in serum and plasma concentrations. Material and Methods: Twenty five adult patients suffering from cholesteatoma (a study group) were included in the study. A comparison group consisted of 25 adult patients admitted to hospital due to nasal septum deviation. MM-9 and TIMP-1 serum and plasma concentrations as well as proteins’ expressions in cholesteatoma tissues (study group) and normal retroauricular skin specimens (control group) were evaluated. MMP-9 and TIMP-1 concentrations were measured using enzyme-linked immunosorbent assay (ELISA). Cholesteatoma tissues and normal retro-auricular skin specimens were evaluated immunohisto-chemically. Results: In the study and comparison groups, MMP-9 and TIMP-1 concentrations were similar with no significant difference within the groups. In cholesteatoma tissues, the expression of the investigated enzyme and its inhibitor was higher than in normal skin specimens, limited mostly to cholesteatoma perimatrix. Conclusion: Cholesteatoma may be limited to the middle ear or parts of the temporal bones. Our findings suggest better clinical usefulness of MMP-9 and TIMP-1 expression in cholesteatoma tissues than either serum or plasma levels of these proteins. It might suggest that the higher the expression of MMP-9 the stronger the inflammation -accompanied cholesteatoma. Histol Histopathol 31, 307-315 (2016)

Key words: Cholesteatoma, Matrix metalloproteinase 9, Tissue inhibitor of metalloproteinase, Serum, Plasma

DOI: 10.14670/HH-11-677