HISTOLOGY AND HISTOPATHOLOGY

Cellular and Molecular Biology

 

Detection of galectin-3 and localization of advanced glycation end products (AGE) in human chronic skin wounds

Daniel Pepe1, Christopher G. Elliott1, Thomas L. Forbes2 and Douglas W. Hamilton1,3

1Department of Anatomy and Cell Biology, 3Division of Oral Biology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada and 2Division of Vascular Surgery, London Health Sciences Center, Victoria Hospital, London Ontario, Canada.

Offprint requests to: Douglas W. Hamilton, Division of Oral Biology, Schulich School of Medicine and Dentistry, Dental Sciences Builiding, The University of Western Ontario, London, Ontario, Canada N6A 5C1. e-mail: dhamil2@uwo.ca


Summary. The matricellular protein galectin-3 (Gal-3) is upregulated in excisional skin repair in rats where it has been shown to modulate the inflammatory phase of repair. Recent research into kidney pathology has implicated Gal-3 as a receptor for advanced glycation end products (AGE), resulting in the binding and clearance of these molecules. AGEs are thought to contribute to defective skin repair in diabetic patients as well as a result of the normal aging process. However, the distribution and localization of Gal-3 and AGEs has never been performed in human chronic skin wound tissue. Using immunohistochemistry, the localization of Gal-3 and AGEs in tissue isolated from chronic wounds and non-involved skin from the same patient was investigated. Of the 16 patients from which tissue was isolated, 13 had type II diabetes, one had type I diabetes and 2 patients without diabetes were also examined. In non-involved dermis, Gal-3 was detected strongly in the epidermis and in the vasculature. However, at the wound edge and in the wound bed, the level of Gal-3 labelling was greatly reduced in both the epidermis and vasculature. Labelling of serial sections for Gal-3 and AGE demonstrated that where Gal-3 immunoreactivity is reduced in the epidermis and vasculature, there is a concomitant increase in the level of AGE staining. Interestingly, similar labelling patterns were evident in diabetic and non-diabetic patients. The results from our study demonstrate an inverse correlation between Gal-3 and AGEs localization, suggesting that Gal-3 may protect against accumulation of AGEs in wound healing
. Histol Histopathol 29, 251-258 (2014)

Key words: Advanced glycation end product, Chronic wounds, Diabetes, Galectin-3, Wound repair

DOI: 10.14670/HH-29.251