Differential expression of proteins related to smooth muscle cells and myofibroblasts in human thoracic aortic aneurysm

Amalia Forte¹, Alessandro Della Corte^1,2, Mario Grossi^1,3, Ciro Bancone², Ciro Maiello⁴, Umberto Galderisi¹ and Marilena Cipollaro¹

Deptartments of ¹Experimental Medicine and ²Cardiothoracic and Respiratory Sciences, Second University of Naples, Naples, Italy, ³Department of Experimental Medical Science, Lund University, Lund, Sweden and ⁴Department of Cardiovascular Surgery and Transplant, V. Monaldi Hospital, Naples, Italy.

Offprint requests to: Amalia Forte, Dip. di Medicina Sperimentale, Sezione di Biotecnologie e Biologia Molecolare, Seconda Univeristà degli Studi di Napoli, Via L. de Crecchio, 7, 80138 Napoli, Italy. e-mail: amalia.forte@unina2.it


Summary. Objectives: Increasing knowledge is required for a better comprehension of the etiology of thoracic aortic aneurysm (TAA). The aim of this study was to highlight the modulations in vascular cell phenotypes, including myofibroblasts (MFs), in human TAA specimens compared to healthy aortas.
Methods: histology, RT-PCR and immunohisto-chemical analysis of a panel of molecules, including ED-A Fibronectin (Fn), smoothelin, CD34 and alpha-smooth muscle actin (alpha-SMA), selected on the basis of their informative potential as markers of smooth muscle cells (SMCs) and MF phenotypic modulation, were performed on all samples.
Results: The media of TAAs was characterized by the absence of smoothelin, the unaltered expression of alpha-SMA accompanied by an alteration of its distribution pattern, and by the activated expression of the ED-A isoform of Fn.
We found a concentration of round-shaped cells exclusively in the adventitia and in the perivascular tissue of TAAs, also rich in vasa vasorum, largely expressing alpha-SMA, while a sub-population also expressed ED-A Fn and CD34.
CD34 was expressed by several cells in the intima of TAAs, together with cells expressing cytoplasmatic ED-A Fn and alpha-SMA in comparison to healthy aortas.
Conclusion: TAA specimens show an altered expression and localization of SMC and MF differentiation markers in comparison to healthy aortas, with possible implications on remodeling. Histol Histopathol 28, 795-803 (2013)

Key words: Thoracic aortic aneurysm, Myofibroblasts, Fibronectin, CD34, Smoothelin, Alpha-smooth muscle actin

DOI: 10.14670/HH-28.795