Expression of receptor for hyaluronan-mediated motility (RHAMM) in ossifying fibromas
Hiroko Hatano1, Ikuko Ogawa2, Hideo Shigeishi1, Yasusei Kudo3, Kouji Ohta1, Koichiro Higashikawa1, Masaaki Takechi1, Takashi Takata4 and Nobuyuki Kamata1
1Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi, Minami-ku, 2Center of Oral Clinical Examination, Hiroshima University Hospital, 3Department of Oral Molecular Pathology, Institute of Health Biosciences, The University of Tokushima, Tokushima and 4Department of Oral and Maxillofacial Pathobiology, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Offprint requests to: Nobuyuki Kamata, Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan. e-mail: nokam@hiroshima-u.ac.jp
Summary. Fibro-osseous lesions of the jaw are poorly understood because of a significant overlap of clinical, radiological and histological features among the various types, though they present distinct patterns of disease progression. An ossifying fibroma is associated with significant cosmetic and functional disturbances, as it shows expansive proliferation. Thus, it is important to establish a specific marker, as well as clearly elucidate its etiology for diagnosis and proper treatment.
We previously established immortalized cell lines from human ossifying fibromas of the jaw and found that they highly expressed the receptor for hyaluronan (HA)-mediated motility (RHAMM). In this study, we examined the expression of RHAMM mRNA in 65 fibro-osseous lesions, including ossifying fibroma, fibrous dysplasia and osseous dysplasia, as well as 5 normal jaws, using real-time RT-PCR and immunohisto-chemistry assays. RHAMM mRNA and protein expression were significantly elevated in the ossifying fibroma specimens.
These results suggest that detection of upregulated RHAMM expression in an ossifying fibroma assists with differential diagnosis and has a key role in elucidation of its pathophysiology. Histol Histopathol 28, 473-480 (2013)
Key words: Fibro-osseous lesion, Ossifying fibroma, RHAMM
DOI: 10.14670/HH-28.473