Cellular and Molecular Biology


Expression of Histone Deacetylases 1, 2 and 3 in histological subtypes of testicular germ cell tumours

Florian R. Fritzsche1*, Anja Hasler2*, Peter K. Bode1, Heiner Adams1, Hans. H. Seifert3, Tullio Sulser2, Holger Moch1, André Barghorn4* and Glen Kristiansen1*

1Institute of Surgical Pathology, 2Division of Urology, University Hospital Zurich, Switzerland, 3Department of Urology, Hegau-Bodensee-Klinikum, Singen, Germany and 4Institute of Pathology - Medica, Zurich, Switzerland
*Equal contribution.

Offprint requests to: Florian R. Fritzsche, MD, Institute of Surgical Pathology, University Hospital Zurich, Schmelzbergstrasse 12, 8091 Zurich, Switzerland. e-mail: florian.fritzsche@usz.ch

Summary. In this study we aimed to evaluate the protein expression of class I histone deacetylases (HDAC) in testicular germ cell tumours (GCT) and to analyse differences between the histological subtypes of testicular GCT. 325 testicular GCT were included in a tissue microarray with each histological subtype of the tumour being separately represented on this array. Expression of class I HDAC isoforms 1, 2 and 3 was assessed by immunohistochemistry.
While HDAC2 and 3 were highly expressed in all histological subtypes of GCT, HDAC1 was almost consistently expressed at lower levels. We observed significant differences in the expression of the respective HDACs between seminoma and non-seminoma GCT tissue components. Interestingly, choriocarcinomas showed generally high expression values for all three class I HDAC isoforms. Relevant correlations with clinicopathological parameters could not be demonstrated.
Contrasting published findings on other tumour entities, no immediate practical diagnostic or prognostic value for HDAC1-3 in GCT could be inferred. However, the high expression levels might still be indicative for a treatment response to HDAC inhibitors which ought to be evaluated in further studies
. Histol Histopathol 26, 1555-1561 (2011)

Key words: HDAC, Immunohistochemistry, Germ cell tumour, Seminoma, Embryonal carcinoma

DOI: 10.14670/HH-26.1555