HISTOLOGY AND HISTOPATHOLOGY

Cellular and Molecular Biology

 

Comparison of the tumorigenic potential of liver and kidney tumors induced by N-nitrosodimethylamine

Gyorgy Trencsenyi1, Tamas Juhasz2, Fruzsina Bako1, Terez Marian3, Istvan Pocsi1, Pal Kertai4, Janos Hunyadi5 and Gaspar Banfalvi1

1Department of Microbial Biotechnology and Cell Biology, 2Department of Anatomy, Histology and Embryology, 3Institute of Nuclear Medicine, 4Institute of Preventive Medicine and 5Department of Dermatology, University of Debrecen, Debrecen, Hungary.

Offprint requests to: Prof. Gaspar Banfalvi PhD, DSc, Deprtment of Microbial Biotechnology and Cell Biology, University of Debrecen, 1 Egyetem Square, Debrecen 4010 Hungary. e-mail: bgaspar@delfin.klte.hu


Summary. The aim of the study was to determine the tumorigenic potential of two cell lines established from N-nitrosodimethylamine induced rat hepatocarcinoma (HeDe) and mesenchymal renal tumors (NeDe). The basis of the distinction is that human cancers are known to overexpress facilitative GLUT transporters and TGF-ß1 protein. These proteins are linked to the increased metabolic energy consumption indicating uncontrolled growth and proliferation. We have assayed not only the expression of GLUT-1, GLUT-3 and TGF-ß1 proteins, but also the uptake of 2-fluoro-[18F]-2-deoxi-D-glucose (18FDG), a tracer for cancer diagnosis. Western blot analysis and whole body autoradiography were used to measure the 18FDG uptake of tumor cells. Elevated 18FDG uptake was measured in both tumor cell lines. Whole body autoradiography provided evidence that the uptake of 18FDG was lower in the necrotic inner part than in the more vascularized outer parts of primary hepatocarcinoma and mesenchymal renal tumors. GLUT-1 overexpression in hepatocarcinoma tumor, and high levels of GLUT-3 were found in the NeDe cell line and in the mesenchymal renal tumor. TGF-ß-1 was overexpressed in hepatocarcinoma and mesenchymal renal tumors. In vitro and in vivo parameters support the view that the tumorigenic potential of cancer cells cannot be determined by the expression of a single parameter such as the expression of either GLUT-1, GLUT-3 or 18FDG uptake. Besides the tumorigenic potential of the hepatocarcinoma, the high metabolic activity of the renal tumor indicated by its 18FDG uptake, GLUT-3 and TGF-ß1 expression, the mesenchymal renal tumor induced by N-nitroso-dimethylamine is not a benign, but an an aggressive renal carcinoma
. Histol Histopathol 25, 309-320 (2010)

Key words: Chemical induction, Primary tumor, Tumor cell lines, Gene expression, GLUT transporters, 18FDG uptake, Whole body autoradiography

DOI: 10.14670/HH-25.309