HISTOLOGY AND HISTOPATHOLOGY

Cellular and Molecular Biology

 

Useful DNA typing using AmpFιSTR® Identifiler® Kit for formaldehyde-fixed paraffin-embedded (FFPE) tissues in early gastric cancer patient with lymph node metastasis

Hisako Motani-Saitoh1, Hiroyuki Inoue1, Tohru Tanizawa2, Yoshihiro Nabeya3, Daisuke Yajima1, Mutsumi Hayakawa1, Yayoi Sato1, Yukio Nakatani2, Hisahiro Matsubara3 and Hirotaro Iwase1

1Department of Legal Medicine, Graduate School of Medicine, Chiba University, Inohana, Chuo-ku, Chiba, Japan, 2Department of Diagnostic Pathology, Graduate School of Medicine, Chiba University, Inohana, Chuo-ku, Chiba, Japan and 3Department of Frontier Surgery, Graduate School of Medicine, Chiba University, Inohana, Chuo-ku, Chiba, Japan.

Offprint requests to: Hisako Motani, Department of Legal Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. e-mail: hms1466@faculty.chiba-u.jp


Summary. After distal gastrectomy in a patient with early gastric cancer, 27 regional lymph nodes around the stomach were evaluated for the existence of metastasis. There was a 0IIa+IIc type tumor 2.0x1.5cm in size in the gastric angle of the lesser curvature according to the Japanese Classification of Gastric Carcinoma (JCGC). Histologically, the lesion extended no deeper than the muscularis mucosae. The cancer stage was so early that no metastasis was expected to occur but a lymph node with metastasis was found in one lymph node along the common anterior hepatic artery (station No.8a). This histological type was a little different from that of a primary tumor. The doctor began to suspect that the lymph node with metastasis might have been from another patient by mistake. Therefore, DNA typing using the AmpF
ιSTR® Identifiler® kit was performed in formaldehyde-fixed paraffin-embedded (FFPE) tissues: 2 parts of gastric mucosa without cancer, one part of gastric mucosa with cancer, 4 lymph nodes without metastasis, and the lymph node station No.8a with metastasis. STR typing was successful in 6~14 STR loci and amelogenin gene, and the detected STR type was the same in all samples. Compared with the STR type using DNA from the patient’s blood, the lymph node station No.8a was from the same patient. The lymph node with metastasis turned out to be not from another patient. Therefore, we suggest that DNA typing using the AmpFιSTR® Identifiler® Kit for FFPE samples is useful in such clinical cases. Histol Histopathol 24, 1139-1145 (2009)

Key words: DNA typing, AmpFιSTR® Identifiler® Kit, Gastric cancer, Lymph node metastasis, Formaldehyde-fixed paraffin-embedded (FFPE) tissues

DOI: 10.14670/HH-24.1139