HISTOLOGY AND HISTOPATHOLOGY

Cellular and Molecular Biology

 

Expression of the Shwachman-Bodian-Diamond syndrome (SBDS) protein in human pancreatic cancer and chronic pancreatitis

Hany Kayed1, Sandor Bekasi1, Shereen Keleg1, Thilo Welsch1, Irene Esposito3, Akiko Shimamura4, Christoph W. Michalski2, Helmut Friess2 and Jörg Kleeff2

1Department of General Surgery, University of Heidelberg, Heidelberg, Germany, 2Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany, 3Institute of Pathology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany and 4Department of Pediatric Hematology/Oncology, Children’s Hospital Boston and Dana Farber Cancer Institute Boston, MA, USA; Department of Pediatric Hematology/Oncology, University of Washington and Children’s Hospital, Seattle, WA, USA.

Offprint requests to: Jorg Kleeff, MD, Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Ismaningerstrasse 22, 81675 Munich, Germany. e-mail: kleeff@t-online.de


Summary. Background: The Shwachman-Bodian-Diamond syndrome (SBDS) protein is a member of a highly conserved family which influences RNA activation and is associated with pancreatic, skeletal and bone marrow deficiencies, as well as hematological malignancies. Methods: In this study, the expression and localization of SBDS were investigated in normal human pancreatic tissues, chronic pancreatitis (CP) tissues, primary and metastatic pancreatic ductal adenocarcinoma (PDAC) tissues, as well as in cultured pancreatic cancer cell lines by immunohistochemistry, immunoblotting and immunocytochemistry. Results: In the normal pancreas, SBDS was localized in the cytoplasm of islet cells and ductal cells. In CP tissues, SBDS was found in the cytoplasm of ductal cells, tubular complexes, stromal fibroblasts and in PanIN1-2 lesions. In PDAC tissues, SBDS exhibited cytoplasmic and occasionally nuclear localization in tubular complexes, PanIN1-3 lesions, cancer cells, and stromal fibroblasts. Different levels of SBDS protein were detected in cultured pancreatic cancer cell lines. Conclusion: SBDS is expressed in normal, CP, and PDAC tissues, as well as in pancreatic cancer cell lines. The different expression and localization patterns suggest a role of SBDS in the pathogenesis of, or response to, inflammatory and neoplastic pancreatic diseases. Histol Histopathol 23, 819-826 (2008)

Key words: SBDS, Pancreatic adenocarcinoma, Chronic pancreatitis

DOI: 10.14670/HH-23.819