HISTOLOGY AND HISTOPATHOLOGY

Cellular and Molecular Biology

 

Expression profile of metastasis-related genes in invasive oral cancers

Yung-Yen Chiang1, Ming-Hsui Tsai2, Tze-Yi Lin3 and I-Ping Chiang3

1Department of Dental Laboratory Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan and 2Department of Otolaryngology and 3Department of Pathology, China Medical University Hospital, Taichung, Taiwan.

Offprint requests to: Dr. Yung-Yebn Chiang, Department of Dental Laboratory Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan. e-mail: YYChiang668@gmail.com


Summary. We used suppression subtractive hybridization (SSH) and oligonucleotide microarray to differentiate expression profiles of metastasis-related genes and to evaluate their clinical significance in patients with invasive oral cancer (OCa). Overexpression of the specific genes was confirmed by reverse transcription-PCR (RT-PCR). Cells expressing the gene were identified by immunohistochemistry in pathology specimens. Clinical correlation and significance were analyzed statistically. Using these methods, we detected increased expressions of MMP-1, -3, -7, -9, -10 and interleukin (IL)-8 in invasive OCa. Moreover, our data showed that overexpressions of MMP-1, -3, -7, -10 and IL-8 were associated with reduced survival. Histol Histopathol 23, 1213-1222 (2008)

Key words: Oral cancer, Matrix metalloproteinase, Prognosis

DOI: 10.14670/HH-23.1213