Expression of annexin AI in conventional renal cell carcinoma (CRCC) correlates with tumour stage, Fuhrman grade, amount of eosinophilic cells and clinical outcome
U. Zimmermann1, C. Woenckhaus2, S. Teller3, S. Venz3,4, M. Langheinrich3, C. Protzel1, S. Maile5, H. Junker3 and J. Giebel5
1Department of Urology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany, 2Institute for Pathology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany, 3Department of Medical Biochemistry and Molecular Biology, 4Laboratory for Functional Genomics, Ernst Moritz Arndt University Greifswald, Greifswald, Germany and 5Institute for Anatomy, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
Offprint requests to: U. Zimmermann MD, Department of Urology, Ernst-Moritz-Arndt University, Fleischmannstrasse 42-44, 17475 Greifswald, Germany. e-mail: ziuro@uni-greifswald.de
Summary. There is increasing evidence that Annexin AI (ANX AI) expression is dysregulated in several carcinomas and tumour cell lines. In order to gain insight into the putative role of ANX AI in tumorigenesis, clinical outcome and metastatic potential of conventional renal cell carcinomas (CRCCs) we investigated the expression of ANX AI in CRCCs and metastases. Furthermore, it was elucidated whether ANX AI overexpression affects migratory potential in Caki-1 cells.
ANX AI immunohistochemistry was performed on 33 samples of CRCCs and 10 metastases. ANX AI expression was assessed in 12 samples by 2-dimensional gelelectrophoresis (2-DE), subsequent mass spectrometry and RT-PCR. Immunohistochemical data were statistically correlated with pathological parameters, amount of eosinophilic cells and clinical outcome. Furthermore, a haptotactic migration assay was done on Caki-1 cells transfected with ANX AI.
Immunostaining for ANX AI was found in 18 tumours and all metastases investigated. Intensity of immunohistochemical staining correlated to Fuhrman grade, amount of eosinophilic cells and clinical outcome. 2-DE and RT-PCR confirmed the presence of ANX AI in neoplastic tissue. Overexpression of ANX AI did not significantly influence cell migration.
From these findings ANX AI expression seems to be related to Fuhrman grade, clinical outcome and metastatic potential of CRCCs. Thus ANX AI could serve as a prognostic marker for tumour progression. Histol Histopathol 22, 527-534 (2007)
Key words: Annexin AI, Kidney, Cancer, Immunohistochemistry, Cell Migration
DOI: 10.14670/HH-22.527