Immunohistochemical study of TAFII250 in the rat laryngeal nervous system
H. Okano1,2, H. Bamba2, Y. Hisa2, S. Makino3, S. Ando3, G. Tamiya3, S. Goto4, R. Kaji5, H. Kimura1 and I. Tooyama1
1Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatukinowa-cho, Otsu, 2Department of Otolaryngology-Head and Surgery, Kyoto Prefectural University of Medicine, Kawaranachi-Hirokoji, Kyoto, 3Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, 4Department of Neurosurgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, 5Department of Neurology, Graduate School of Medicine, Tokushima University, Tokushima, Japan
Offprint requests to: Ikuo Tooyama, Professor, Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatukinowa-cho, Otsu 590-2192, Japan. e-mail: kinchan@belle.shiga-med.ac.jp
Summary. The cause of spasmodic dysphonia, a dystonic disorder of the larynx, remains unclear. Recently, TAFII250, TATA-box binding protein associated factor, was suggested to be involved in dystonia parkinsonism. There is a possibility that TAFII250 is involved in spasmodic dysphonia, but little information is available about the expression of TAFII250 in the laryngeal nervous system. In this study, we investigated the localization of TAFII250 protein in the rat laryngeal nervous system by immunohistochemistry. TAFII250-immunoreactivity was detected in the nodose ganglion and superior cervical ganglion. In these nuclei, TAFII250 was localized in the nucleus of NeuroTrace-positive neurons but not in GFAP-positive glial cells. No positive cells were detected in the motor and parasympathetic nervous system. TAFII250-immunoreactivity was sustained between 3 and 7 days after vagotomy, but at 14 days expression was down-regulated in the distal part of the nodose ganglion. These findings suggest that TAFII250 plays an important role in the laryngeal innervation of the sensory and sympathetic nervous systems. Histol Histopathol 20, 1029-1035 (2005)
Key words: TAFII250, Laryngeal nervous system, Nodose ganglion, Supura cervical ganglion, Spasmodic dysphonia
DOI: 10.14670/HH-20.1029