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Time of origin of the rat pineal gland cells. A bromodeoxyuridine immunohistochemical study J.L. Calvo, J. Boya, A.L. Carbonell and J.E. García-Mauriño Department of Histology, Faculty of Medicine, Complutense University, Madrid, Spain Offprint requests to: Prof. Jesús Boya Vegue, Department of Histology, Faculty of Medicine, Complutense University , 28040 Madrid, Spain
Summary. The immunohistochemical detection of bromodeoxyuridine (BrdU) was used to study the time of origin of the cells in the pineal gland of the rat. A study was made involving 17 groups of 4 rats each, administered with a single dose of bromodeoxyuridine (BrdU, 25 mg/kg) in 7 phases of the embryonic period (E15 to E21) and in 10 postnatal phases (between P0 and P30), followed by determination in each rat of the number of visible immune-labeled cells in the pineal gland 60 days after birth. The results show that approximately 60% of the pineal cells underwent the last division(s) prior to differentiation in the prenatal period between E18 and E21. The rest of the pineal cells originated after birth, particularly in the first 5 postnatal days. Histol. Histopathol. 19, 137-142 (2004) Key words: Bromodeoxyuridine,
BrdU, Pinealocytes, Pineal gland, Development, Proliferation |