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Time of origin of the rat
pineal gland cells. A bromodeoxyuridine immunohistochemical study
J.L. Calvo, J. Boya, A.L. Carbonell and
J.E. García-Mauriño
Department of Histology, Faculty of Medicine,
Complutense University, Madrid, Spain
Offprint requests to: Prof. Jesús
Boya Vegue, Department of Histology, Faculty of Medicine, Complutense
University , 28040 Madrid, Spain
Summary. The
immunohistochemical detection of bromodeoxyuridine (BrdU) was
used to study the time of origin of the cells in the pineal gland
of the rat. A study was made involving 17 groups of 4 rats each,
administered with a single dose of bromodeoxyuridine (BrdU, 25
mg/kg) in 7 phases of the embryonic period (E15 to E21) and in
10 postnatal phases (between P0 and P30), followed by determination
in each rat of the number of visible immune-labeled cells in
the pineal gland 60 days after birth. The results show that approximately
60% of the pineal cells underwent the last division(s) prior
to differentiation in the prenatal period between E18 and E21.
The rest of the pineal cells originated after birth, particularly
in the first 5 postnatal days. Histol. Histopathol. 19, 137-142
(2004)
Key words: Bromodeoxyuridine,
BrdU, Pinealocytes, Pineal gland, Development, Proliferation
DOI: 10.14670/HH-19.137
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