Cellular and Molecular Biology

Ultrastructural evaluation of the effect of endosulfan on mice kidney

Y. Çaglar1, M. Kaya1, E. Belge2 and U.Ö. Mete1

1Department of Histology-Embryology, Faculty of Medicine, Çukurova University, Adana-Turkey and
2Department of Biochemistry, Faculty of Medicine, Sütçü Imam University, Kahramanmaras, Turkey

Offprint requests to: Dr. Yldz Çaglar, Çukurova Üniversitesi Tp Fakültesi. Histoloji-Embrioloji AB.D., 01330 Balcal, Adana, Turkey. Fax: +90 322 338 6572. e-mail: ycaglar@cu.edu.tr


Summary. In this study, the effect of the endosulfan on mice kidney was investigated at ultrastructural level. Moreover, biochemical analyses (G6PD, CAT, SOD, GSH and MDA) were determined in supernatant of kidney tissue. Endosulfan (13mg/kg/day body weight) was administered orally to mices via intragastric-during 10 days. The presence of mitochondrial degeneration in cytoplasm of proximal convoluted tubule cells were a striking feature. Furthermore, there was lipofuscin granules and membranous structures in some of proximal convoluted tubule cells. In some glomeruli, ultrastructural changes such as fusion in pedicels and focal thickening at glomerular basal membrane were seen. There were cytoplasmic bulges in some distal convoluted tubule cells. The biochemical results of the experimental group were significant when compared to the control. The effect of the endosulfan was mainly on the proximal convoluted tubule cells. Morever, the other parts of the nephron were effected. Thus, this degeneration in kidney may be thought that oxidative stress may play a role to the mediator in changing configuration of cell membrane and seem to account for the morphologic alteration of kidney. Histol. Histopathol. 18, 703-708 (2003)

Key words: Electron microscopy, Endosulfan, Kidney, Nephrotoxicity

DOI: 10.14670/HH-18.703