Immunohistochemical localization of human fructose1,6bisphosphatase in subcellular structures of myocytes
A. Gizak, D. Rakus and A. Dzugaj
Department of Animal Physiology, Institute of Zoology, Wroclaw University, Wroclaw, Poland
Offprint requests to: Dr. A. Dzugaj, Department of Animal Physiology, Institute of Zoology, Wroclaw Unviersity, Cybulskiego 30, 50-205 Wroclaw, Poland. Fax: (48 71) 3759213. e-mail: dzugajan@biol.uni.wroc.pl
Summary. The localization of fructose-1,6-bisphosphatase (FBPase) in human skeletal muscle was determined immunohistochemically using polyclonal antibodies. Light microscopy analysis, confirmed with the use of confocal microscopy, indicated that the enzyme is localized on both sides of the Z line of myocytes. The immunohistochemical investigation was confirmed by a co-sedimentation experiment which revealed that muscle FBPase binds strongly to a-actinin - a major structural protein of the Z line. This is the first report on localization of FBPase in skeletal muscle tissue. Histol. Histopathol. 18, 135-142 (2003)
Key words: Fructose-1,6-bisphosphatase, Confocal microscopy,
Muscle, Immunohistochemistry
DOI: 10.14670/HH-18.135