Lectin-histochemical and -cytochemical study of periodic acid Schiff-Positive lysosome granules as a histological feature of the female mouse kidney

A. Yabuki1, S. Suzuki2, M. Matsumoto1 and H. Nishinakagawa1

1Department of Veterinary Anatomy, Faculty of Agriculture, Kagoshima University, Korimoto Kagoshima-shi, Kagoshima, Japan and 2Institute of Laboratory Animal Sciences, Faculty of Medicine, Kagoshima University, Kagoshima-shi, Kagoshima, Japan

Offprint requests to: Akira Yabuki, Department of Veterinary Anatomy, Faculty of Agriculture, Kagoshima University, 21-24 Korimoto 1, Kagoshima-shi, Kagoshima 890-0065, Japan. Fax: +81-99-285-8710. e-mail: yabu@vet.agri.kagoshima-u.ac.jp

 

Summary. Renal proximal straight tubules (PST) of the female mouse contain periodic acid Schiff­positive lysosome granules. An excellent example of this is found in the kidneys of female DBA/2Cr mice. In the present study, lectin-histochemistry showed that lectin-positive granules occur in the PST of DBA/2Cr mice. Out of twenty-one lectins studied, the granules bound WGA, s-WGA, LEL, STL, DSL, GSL-II, VVL, RCA-I, ECL, PSA, LCA and PHA-E. Such granules were also observed in the proximal convoluted tubules (PCT). In addition, heterogeneous binding to the SBA or DBA was observed in the PST. Lectin-cytochemistry for s-WGA, STL, VVL, RCA-I, ECL and PSA, showed that: 1) lysosomes bind a higher level of s-WGA or STL than VVL, RCA-I, ECL or PSA; 2) PSA binding is similar in PST and PCT; 3) there are many PCT lysosomes that are negative for s-WGA, STL, VVL, RCA-I, and ECL lectin binding; and 4) s-WGA binding is highly specific to the lysosomes of the PST. Based on the binding specificities of each lectin, it was suggested that the mannose content of PST and PCT lysosomes is similar, and that PST lysosomes have a high level of N-acetylglucosamine, N-acetylgalactosamine, galactose or galactosyl (ß1,4) N-acetylglucosamine. Histol. Histopathol. 17, 1017-1026 (2002)

Key words: Female mouse, Kidney, Lectin, Lysosome, Proximal straight tubule

DOI: 10.14670/HH-17.1017