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Abnormalities in dendritic cell and macrophage
accumulation in the pancreas of nonobese diabetic (NOD) mice during
the early neonatal period
S. Charré1, J.G.M. Rosmalen2, C. Pelegri3, V. Alves1,
P.J.M. Leenen2, H.A. Drexhage2 and F. Homo-Delarche1
1CNRS UMR 8603, University Paris V, Necker Hospital, Paris,
France, 2Department of Immunology, Erasmus University, Rotterdam,
The Netherlands and 3Department of Physiology, Division IV, School
of Pharmacy, University of Barcelona, Barcelona, Spain
Offprint requests to: Françoise
Homo-Delarche, CNRS UMR 8603, Hôpital Necker, 161, rue de
Sèvres, 75015 Paris, France. Fax: 33 1 44 49 06 76. e-mail:
fhomodel@wanadoo.fr
Summary. Dendritic cell (DC), macrophage (Mø)
and lymphocyte infiltrations have been observed in normal human
perinatal pancreata, but have never been investigated so early
in control mice. In type 1 diabetes-prone NOD mice, these cells
are thought to infiltrate first the periphery of the islets of
Langerhans around weaning before further islet infiltration and
ß-cell destruction.
We quantified, during the first month of life, the numbers of
DC (characterized by CD11c positivity and dendritic morphology),
histiocyte-like Mø (characterized by ER-MP23 positivity)
and Mø with scavenging potential (characterized by BM8
positivity) in C57BL/6, DBA/2 and BALB/c control, and NOD and
lymphocyte-deficient NODscid mouse pancreata.
First, CD11c+ DC were present at low densities from birth onwards
in control pancreata, while densities were higher in NOD and NODscid.
Second, high numbers of BM8+ and ER-MP23+ Mø were observed
at birth in all strains investigated. After birth, particularly
BM8+ cells disappeared progressively in control strains, but not
in NOD and NODscid. Third, NOD mice also had more ER-MP23+ Mø
at birth compared to controls. Finally, DC and Mø localizations
were similar in all strains, i.e., mostly as dispersed cells in
perivascular, periductular, peri-islet areas and interlobular
septa. The most remarkable finding was that particularly BM8+
Mø, were seen at sites of islet neogenesis and predominantly
at the duct-islet interface.
Our data showed that different types of APC were present in the
pancreas during postnatal development in various control mouse
strains and some differences were observed in NOD and NODscid
mice from birth onwards. Histol. Histopathol. 17, 393-401 (2002)
Key words: Macrophages, NOD, Neonatal pancreas development
DOI: 10.14670/HH-17.393
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