Human lung fructose-1,6-bisphosphatase is localized in pneumocytes II

A. Gizak1, D. Rakus1, J. Kolodziej2, M. Zabel3, A. Ogorzalek4 and A. Dzugaj1

1Department of Animal Physiology, Institute of Zoology, Wroclaw University, Wroclaw, Poland,
2Lower Silesian Pulmonary Center, Department of Thoracic Surgery, Wroclaw, Poland,
3Department of Histology, Wroclaw University of Medicine, Wroclaw, 4Department of Histology, Poznan University of Medicine, Poznan, Poland and 5Department of General Zoology, Institute of Zoology, Wroclaw University, Wroclaw, Poland

Offprint requests to: Dr. Andrzej Dzugaj, Department of Animal Physiology, Institute of Zoology, Wroclaw University, Cybulskiego 30, Wroclaw, Poland. Fax: (48 71) 322-28-17. e-mail: dzugajan@biol.uni.wroc.pl

Summary. The localization of fructose-1,6-bis-phosphatase (Fru-1,6-Pase EC 3.1.3.11) in human alveolar epithelium was determined immunohisto-chemically using a polyclonal antibody raised against the enzyme purified from human liver. The immuno-histochemical analysis revealed that the Fru-1,6-Pase was localized in pneumocytes II and was absent in pneumocytes I. Hypothetically Fru-1,6-Pase participating in glucose-6-phosphate synthesis from noncarbohydrate precursors increases NADPH level which is used for surfactant synthesis and for glutathione redox cycle. Histol. Histopathol. 16, 53-55 (2001)

Key words: Fructose-1,6-bisphosphatase, Immunocyto-chemistry, Lung, Pneumocytes

DOI: 10.14670/HH-16.53