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Human papillomaviruses and DNA ploidy in anal condylomata
acuminata
S. Rihet1, P. Bellaich2, M. Lorenzato1, D.
Bouttens1, P. Bernard2, P. Birembaut1 and C. Clavel1
1Cell Biology Unit, Laboratoire Pol Bouin,
Reims, France and 2Department of Dermatology, C.H.U. de Reims,
France
Offprint requests to: Dr.
Christine Clavel, Laboratoire Pol Bouin, Hôpital Maison
Blanche, 45, rue Cognacq-Jay, 51100 Reims, France. Fax: (33) 326
78 77 39
Summary. Previous
studies have emphasized the usefulness of DNA ploidy measurement
and Human Papillomavirus (HPV) detection as pronostic markers
in low grade cervical lesions. We addressed the eventual relationship
between HPV type, DNA profile, and p53 tumor suppressor protein
expression in anal condylomata acuminata to eventually determine
parameters which may be considered as predictive risk factors
for the development of cancer. DNA ploidy was assessed by image
cytometry after Feulgen staining of contiguous serial sections
of 45 anal condylomata acuminata without atypia containing HPV
detected by in situ hybridization and Polymerase Chain Reaction
(PCR). p53 expression was detected by immunohistochemistry. DNA
aneuploidy was found in 53.3% of these lesions, 48.9% containing
non oncogenic HPV types 6 and/or 11 and 4.4% harbouring HPV types
11 and 18. The DNA diploid lesions were all associated with non
oncogenic HPV types 6 and/or 11 and one case also contained HPV
type 33. There was no significant correlation between the detection
of DNA aneuploidy and the presence of immunodetected p53. DNA
aneuploidy was not related to the presence of oncogenic HPV in
anal condylomata acuminata. The DNA aneuploid profile frequently
observed, especially in lesions associated with non oncogenic
HPV types, is not yet well explained and cannot be considered
as a prognostic factor. In contrast, a more intensive clinical
follow-up should be proposed in patients with oncogenic HPV associated
to DNA aneuploidy. Histol. Histopathol. 15, 79-84 (2000)
Key words: Human
papillomavirus, Anal condylomata acuminata, DNA ploidy
DOI: 10.14670/HH-15.79
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