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Heart mitochondria in rats submitted to chronic hypoxia
J. Cervós Navarro1, R.Ch. Kunas2, S.
Sampaolo2 and U. Mansmann2
1Instituto de Ciencias Neurológicas
y Gerontológicas, Universitat Internacional de Catalunya,
Barcelona, Spain, 2Department of Neuropathology and 3Department
of Medical Statistic and Epidemiology, Freie Universität
Berlin, Hindenburgdamm, Berlin, Germany
Offprint requests to:
Prof. Dr. J. Cervós-Navarro, Instituto de Ciencias Neurológicas
y Gerontológicas, Universitat Internacional de Catalunya,
c/Inmaculada 22, 08017 Barcelona, Spain. Fax: +34 93 434 0608
Summary. The
effect of prolonged exposure to normobaric hypoxia on the mitochondria
of myocard of rats exposed for several weeks to 8 and 7% O2 has
been morphometrically evaluated. Twelve male Wistar rats housed
in Nalgene cages (2 per cage) with a batch of six cages placed
in plexiglass chambers were maintained in air/N2 mixtures containing
different concentrations of O2. Six animals kept in similar cages
under normoxia served as controls. When at day 60 the FIO2 was
reduced to 8%, the weight increase stagnated and after the 81st
test day, on which the hypoxic animals were subdivided into 8%
and 7% groups the weight curve showed a decrease in the mean body
weight for both groups. The arrest and the following loss of weight
beyond the 85th day may be interpreted as the expression of a
limit reached in the compensation capacity. In the 8%-group the
shape of the mitochondria varied more markedly often with budding
and furrowing of the surface. In the 7%-group bizarre shapes and
wide variations in size with a decided shift towards larger mitochondria
were noteworthy. While rats kept under 8% oxygen exhibited a numerical
increase in myocardial mitochondria compared to controls, the
mitochondria of the 7%-group were numerically reduced. The results
suggest that hypoxia of 8% oxygen is compensatable, if only to
some extent, by an increasing surface of mitochondrial membranes,
and that further reduction of oxygen causes compensation mechanisms
to fail as seen by the severe alterations of the mitochondrial
population of the cardiomyocyte in the 7%-group. Histol. Histopathol.
14, 1045-1052 (1999)
Key words: Heart,
Mitochondria, Chronic hypoxia, Ultrastructural morphometry
DOI: 10.14670/HH-14.1045
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