Cellular and Molecular Biology


Tissue transfers: Substrates for cytology and cytochemistry of animal tissues

J.M.D. McDonald1, J.R. Matyas1, J.P. Rattner2 and J.B. Rattner2

1Departments of Pathology and Laboratory Medicine, 2Cell Biology and Anatomy,
3Biochemistry and Molecular Biology, The University of Calgary Health Sciences Centre, Calgary, Alberta, Canada

Offprint requests to: J.B. Rattner, Department of Cell Biology and Anatomy, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1. e-mail: rattner@acs.ucalgary.ca


Summary. The Tissue Transfer Technique (TTT) is a novel method of sampling animal tissue that can be used to study tissue morphology, chemistry and physiology. This review provides an overview of the technique and demonstrates its use to detect the tissue distribution of specific epitopes, lectin binding sites and nucleic acids as well as its application as an organ monolayer in culture. These applications are compared and contrasted with standard histological techniques including the "Tissue Printing Technique" developed to sample plant tissue. Histol. Histopathol. 14, 1341-1346 (1999)

Key words: Tissue transfer, Immunocytochemistry, Cytochemistry

DOI: 10.14670/HH-14.1341