Synaptophysin immunoreactivity in the cat cochlear nuclei

P. Gil-Loyzaga1, M.V. Bartolomé1 and A. Ibáñez2

1Department of Surgery II (ORL), Faculty of Medicine and Center of Cell Culture (CAI-UCM) and 2Department of Cell Biology, Faculty of Medicine, Complutense University of Madrid, Madrid, Spain

Offprint requests to: Prof. P. Gil-Loyzaga MD., PhD., Apartado de Correos 60075, 28080 Madrid, Spain

Summary. The presence of synaptophysin, a presynaptic Ca2+ binding glycoprotein, has been analyzed in the cochlear nucleus complex of the adult cat using an anti-synaptophysin monoclonal antibody. Synaptophysin immunoreactivity was differently distributed between regions of cochlear nuclei. Terminal boutons contacting directly with neurons (cell bodies and dendrites) or in the neuropil of ventral and dorsal cochlear nuclei appeared immunostained. In the ventral cochlear nucleus, synaptophysin-labelled boutons were clearly defined in contact with spherical, globular, multipolar, octopus and cochlear-nerve root neurons. However, the dorsal cochlear nucleus showed a dense immunostained network of synaptophysin-labelled puncta, inside which some neuron cell bodies were observed. The present findings show a differential distribution of synapto-physin-immunostained boutons between the ventral and the dorsal cochlear nuclei of the cat. The present description of the different synaptophysin-labelled terminal boutons in the cochlear nuclei complex and their pattern of distribution, will be useful for further studies on development, degeneration or regeneration of the peripheral auditory pathway. Histol Histopathol 13, 415-424 (1998)

Key words: Cochlear nuclei, Synaptophysin, Auditory pathway, Cat

DOI: 10.14670/HH-13.415