HISTOLOGY AND HISTOPATHOLOGY

From Cell Biology to Tissue Engineering

 

Ezrin and moesin expression in canine and feline osteosarcoma

Juraj Hlavaty1, Birgitt Wolfesberger2, Marlene Hauck3, Barbara Obermayer-Pietsch4, Andrea Fuchs-Baumgartinger5, Ingrid Miller6 and Ingrid Walter1,7

1Institute of Anatomy, Histology and Embryology, 2Clinic for Companion Animal Medicine, Unit for Internal Medicine, University of Veterinary Medicine, Vienna, Austria, 3Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA, 4Division of Endocrinology and Metabolism, Department of Internal Medicine, Medical University of Graz, Graz, 5Institute of Pathology and Forensic Veterinary Medicine, 6Institute of Medical Biochemistry and 7VetCORE Facility for Research, University of Veterinary Medicine, Vienna, Austria

Offprint requests to: Ingrid Walter, Institute of Anatomy, Histology and Embryology, University of Veterinary Medicine, 1210 Vienna, Austria. e-mail: Ingrid.Walter@vetmeduni.ac.at


Summary. Biological features of canine osteosarcomas (OS) differ markedly from those found in feline and resemble more human osteosarcomas, in particular for their high rate of metastasis and poor prognosis. Ezrin, radixin and moesin are members of the ERM protein family and link the actin cytoskeleton with the cell membrane. Ezrin and moesin have been shown to be of prognostic significance in tumor progression due to their role in the metastatic process. The objective of this study was to analyze ezrin and moesin protein expression in a series of dog (n=16) and cat (n=8) osteosarcoma samples using immunohistochemistry and western blot techniques. We found that cat OS have a higher moesin expression compared to dog OS, however, the active phosphorylated forms of moesin and ezrin Tyr353 were more abundant in the dog samples. A statistically significant difference was found for the low and high immunohistochemical scores of ezrin and pan-phospho-ERM proteins between cat and dog. Although phospho-ezrin Thr567 was higher in feline OS, the membranous localization in dog OS samples indicates the presence of the biologically active form. Therefore, the observed differences in phosphorylated forms of ezrin and moesin status should be further studied to demonstrate if they are relevant for different biological behavior between dog and cat OS. Histol Histopathol 32, 805-816 (2017)

Key words: Cat, Dog, Osteosarcoma, Ezrin, Moesin, Western blot

DOI: 10.14670/HH-11-848