Metallothioneins and trace elements dyshomeostasis induced by exposure to gasoline vapor in mice
Damir Grebić1*, Marin Tota2*, Hrvoje Jakovac1*, Dalibor Broznić2, Jelena Marinić2, Gordana Canadi2, Čedomila Milin1 and Biserka Radošević-Stašić1
1Department of Physiology and Immunology, and 2Department of Chemistry and Biochemistry, Medical School, University of Rijeka, Rijeka, Croatia
*D. Grebić, M. Tota and H. Jakovac contributited equally to this work.
Offprint requests to: Biserka Radosevic-Stasic, Department of Physiology and Immunology, Medical School, University of Rijeka, Rijeka, Croatia. e-mail: email@example.com
Summary. To investigate the effects of air pollution related with the gasoline/petrochemical industry the expression of metallothionein I (MT-I) mRNA and tissue metals were analyzed in organs of mice, exposed to gasoline (G) vapor in laboratory conditions. Control groups consisted of intact mice and of those exposed in the metabolic chamber to fresh air. The data obtained by RT-PCR and inductively coupled plasma spectrometry have shown that exposure to G vapor leads to upregulation of MT-I mRNA in organs that receive a strong respiratory and olfactory input or participate in gasoline degradation and elimination (lungs, brain, kidney and liver). Besides, in the brain and in the lungs, kidney and liver a decreased tissue content of Zn2+ or Cu2+ and Mg2+ was found (p<0.001). Some of these changes were obtained also in mice closed in the metabolic chamber, pointing to the involvement of stress-induced mechanisms in the transcriptional regulation of MTs. Histol Histopathol 29, 407-416 (2014)
Key words: Gasoline inhalation, Stress, Metallothioneins I, Zinc, Copper, Magnesium, Brain, Lungs, Kidney, Liver