HISTOLOGY AND HISTOPATHOLOGY

Cellular and Molecular Biology

 

BCL10 expression and localization in Ocular Adnexa MALT lymphomas: a comparative cytogenetic and immunohistochemical study

Margherita Cerrone1*, Francesca Collina1*, Anna De Chiara1, Gaetano Corazzelli4, Maria Pia Curcio1, Amalia De Renzo3, Filippo Russo4, Monica Cantile1, Stefania Staibano2, Diego Strianese5, Fausto Tranfa5, Gerardo Botti1, Gaetano De Rosa2 and Renato Franco1

1Pathology Unit, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy, 2Biomorphological and Functional Sciences Department, “Federico II” University, Naples, Italy, 3Hematology Institute, “Federico II” University, Naples, Italy, 4SC, Hematology, "Istituto Nazionale Tumori Fondazione Giovanni Pascale"-IRCCS, Naples, Italy and 5Ophthalmology Department, “Federico II” University, Naples, Italy
*Equal contributors

Offprint requests to: Dr. Renato Franco, Pathology Unit, Istituto Nazione dei Tumori "Fondazione Giovanni Pascale", Via Mariano Semmola, 80131 Naples, Italy. e-mail: r.franco@istitutotumori.na.it


Summary. T(1;14) (p22;q32) involving BCL10 and IGH genes is a rare but recurrent chromosomal aberration in MALT-type lymphoma. It is rarely described in ocular adnexa B cell lymphomas, although nuclear BCL10 shuttling seems to be critical for disease progression in this district. We have evaluated the translocations MALT lymphoma-related in a series of 45 ocular adnexa cases, focusing in particular on their relation with BCL10 expression and its cellular topographic distribution. A prognostic tissue microarray (TMA) with ocular adnexa MALT lymphomas was designed. A study of BCL10 expression and its topographic distribution was performed through immunohistochemistry. In addition the assessment of t(14;18) (q32;q21), t(1;14) (p22;q32) and t(11;18) (q21;q21) was determined by Fluorescent In Situ Hybridization (FISH).
Our series revealed t(14;18) (q32;q21) in 6/43 cases (14,3%). t(1;14) (p22;q32), never described in ocular adnexa MALT lymphomas, was observed in 3/31 (9,7%), two of which exhibited the gain of 3’ upstream BCL10 gene signal (4%), whereas no case showed t(11;18) (q21;q21). Moreover, BCL10 expression was observed in 18/45 cases. In particular its nuclear expression was revealed in 12/45 cases, cytoplasmic expression in 5/45 and both cytoplasmic and nuclear expression in 1/45. Statistical analysis demonstrated that while BCL10 cytoplasmic expression is significantly related to the presence of the investigated chromosomal aberrations, in particular with t(14;18) (q32;q21), BCL10 nuclear shuttling does not show any correlation with these translocations. Our data support that BCL10 nuclear distribution is neither related to BCL10 rearrangement nor to other known translocations
. Histol Histopathol 29, 77-87 (2014)

Key words: BCL10, Genetic aberration, MALT lymphoma, Ocular Adnexal B-cell Lymphoma

DOI: 10.14670/HH-29.77