HISTOLOGY AND HISTOPATHOLOGY

Cellular and Molecular Biology

 

Histological changes in connective tissue of rat tails after bipolar radiofrequency treatment

E. Beltrán-Frutos1, C.M. Bernal-Mañas1, S. Navarro4, A. Zuasti1, C. Ferrer1, M. Canteras2, V. Seco-Rovira1, A.J. García-Collado3 and L.M. Pastor1

1Department of Cell Biology and Histology, Aging Institute, Medical School, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, Murcia, Spain, 2Department of Statistics, Medical School, University of Murcia, Murcia, Spain, 3Polytechnic University School, Engineering Degree in Telecommunication Systems, San Antonio Catholic University, Murcia, Spain and 4TAHE I+D. Polígono de San Gines Industrial Oeste, San Gines, Murcia, Spain.

Offprint requests to: Prof. Dr. Luis M. Pastor, Department of Cellular Biology and Histology, School of Medicine, University of Murcia, Espinardo, 30100 Murcia, Spain. e-mail: bioetica@um.es


Summary. Radiofrequency (RF) has been included in the techniques used in aesthetic surgery/medicine. To date, no studies have performed a histological assessment of changes in the tissue after application of bipolar radiofrequency (BRF) with low energy and frequency. The aim of this study was to examine changes that are produced in connective tissue, principally in the fibroblasts, following BRF treatment. Four groups of rats received a different number of RF sessions (1, 2, 3 and 5). The following parameters were determined: the number of fibroblasts/unit area (FA), the proliferation index (PI), the Heat shock Protein 47 index (HSPI) and the percentage of connective tissue (PC). For statistical analysis, two subgroups (A and B) were made for the variables FA, PI and PC, and another two subgroups (C and D) for the variable HSPI. Significant differences for FA, PI and PC were observed between subgroups A and B, FA and PI having higher values in A, while PC had higher values in B. The HSPI in subgroup C showed significantly higher values than in D. Low energy and frequency BRF led to an increase in the number, proliferation and biosynthetic activity of fibroblasts. The resulting stress suffered by fibroblasts as a result of heat may be associated with the phenomenon of hormesis
. Histol Histopathol 27, 1231-1237 (2012)

Key words: Fibroblast, Radiofrequency, Hormesis, HSP47, Proliferation

DOI: 10.14670/HH-27-1231