Sulfur dioxide restores calcium homeostasis disturbance in rat with isoproterenol-induced myocardial injury
Shanshan Chen1*, Junbao Du1,2*, Yinfang Liang1, Rongyuan Zhang1, Chaoshu Tang2,3 and Hongfang Jin1
1Department of Pediatrics, Peking University First Hospital, Beijing, China, 2Key Laboratory of Molecular Cardiology, Ministry of Education, Beijing, China and 3Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing China
*Shanshan Chen and Junbao Du contributed equally to the research.
Offprint requests to: Hongfang Jin, Department of Pediatrics, Peking University First Hospital, Xi-An Men Str. No. 1, West District, Beijing 100034, China, e-mail: email@example.com
Summary. Backgrounds: sulfur dioxide (SO2) could relieve isoproterenol (ISO)-induced myocardial injury, while the mechanism is unclear. This study aims to explore whether the protective effect of SO2 on ISO-induced myocardial injury was mediated by the restoration of calcium homeostasis disturbance in cardiomyocyte. Methods and results: Rats were randomly divided into four groups: ISO group, ISO+SO2 group, control group and SO2 group. Content of Ca2+ in H9c2 cells was assayed using confocal microscope, and cardiac function parameters were measured by echocardiography. Plasma biochemical values and myocardial ultra-structure changes were measured. Meanwhile, the activity, protein and gene levels of sarcoplasmic reticulum Ca2+ ATPase (SERCA), and protein and phosphorylation of phospholamban (PLN) were detected. We found SO2 derivatives could restore the decreased cardiac function, the abnormal lactate dehydrogenase, creatine kinase, alpha-hydroxybutyrate dehydrogenase, potassium, calcium, blood urea nitrogen and the damaged myocardial ultra-structure in rats, and regulate the increased Ca2+ content in H9c2 induced by ISO. In addition, compared with ISO group, the decreased activities, protein and mRNA level of SERCA, as well as the decreased protein phosphorylation level of PLN in myocardial tissues were increased in ISO+SO2 group. Conclusion: SO2 derivatives might relieve calcium overload in association with the upregulating expression of SERCA and p-PLN/PLN by myocardial tissues in rats with ISO-induced myocardial injury. Histol Histopathol 27, 1219-1226 (2012)
Key words: Sulfur dioxide, Isoproterenol, Myocardial injury, Calcium homeostasis