Cellular and Molecular Biology


TGF-ß1 and VEGF after fresh frozen bone allograft insertion in oral-maxillo-facial surgery

L.F. Rodella1*, G. Favero1*, R. Boninsegna1,2, A. Borgonovo2, R. Rezzani1 and F. Santoro2

1Department of Biomedical Science Biotechnology Section of Human Anatomy, University of Brescia, Brescia, Italy, 2Unit of Oral Surgery, Department of Dentistry and Stomatology, ICP Hospital, University of Milan, Milan, Italy
*These authors equally contribuited to this work.

Offprint requests to: Luigi F. Rodella, Department of Biomedical Sciences and Biotechnology, Viale Europa 11, 25124 Brescia, Italy. e-mail: rodella@med.unibs.it

Summary. Bone regeneration technique using allografts is widely used in oral surgery to repair alveolar defects and to increase alveolar volume for endosseous implant insertions. Bone allografts promote the reabsorption and neo-synthesis of bone tissue, which are regulated by numerous cytokines, proteins and growth factors. In this study, six patients with insufficient alveolar volume for endosseous implant insertions, were treated with bone regeneration technique using Fresh Frozen Bone (FFB) allografts collected from the femoral head or the hip. Samples of bone graft collected during graft insertion surgery and biopsies collected six months later during implantology were fixed, decalcified and analyzed histomorphologically and morphometrically by haematoxylin-eosin staining. In addition, TGF-ß1 and VEGF were analyzed by immunohistochemistry.
The histological analysis of FFBs showed wide areas of calcified bone organized in osteons intermingled with areas of non-calcified matrix containing osteoblasts. However, the regenerated alveolar bone, collected six months after the graft insertion surgery, showed wide areas of non-calcified matrix. TGF-ß1 and VEGF were less expressed in FFB than in regenerated alveolar bone
. Histol Histopathol 25, 463-471 (2010)

Key words: Fresh Frozen Bone (FFB), Bone regeneration, TGF-ß1, VEGF