Analysis of the molecular expression profile of non small cell lung carcinoma associated to chronic obstructive pulmonary disease
Ricardo Garcia-Lujan1, Esther Conde-Gallego2, Fernando Lopez-Ríos3, Jose Luis Martin de Nicolas4, Montserrat Sanchez-Céspedes5, Cristina García-Quero1, Jose Maria Echave-Sustaeta1 and Angel Lopez-Encuentra1
Department of 1Pneumology, 3Pathology and 4Thoracic Surgery Hospital Universitario 12 de Octubre, 2Laboratorio de Dianas Terapeúticas Hospital Madrid Norte Sanchinarro and 5Department of Molecular Pathology CNIO, Madrid, Spain.
Offprint requests to: Ricardo García Luján, Hospital Universitario 12 de Octubre, Avda Cordoba s/n, 28041 Madrid, Spain. e-mail: rglujan@hotmail.com
Summary. Chronic obstructive pulmonary disease (COPD) is an independent risk factor to develop lung cancer but there are no different functional clusters of biomarkers between patients with non-small cell lung cancer (NSCLC) with or without COPD. To analyse protein expression, in order to find out whether samples of resected NSCLC from patients with COPD present a different molecular expression. Observational, cohort, concurrent study with sampling since treatment of disease in patients with NSCLC in initial stages (pIA-pIIB) treated surgically in our hospital between October 1993 and September 1997. The study consisted of the elaboration of tissue arrays with samples from resected tumor, using immunohistochemistry as a study method. Univariate analysis and logistic regression analysis were performed in order to determine molecular markers that showed a differential expression in NSCLC of the patients with COPD. We studied thirty-two proteins in 146 patients. 30% of the patients had COPD. Univariate analysis in patients with COPD showed one molecular marker to be overexpressed and five molecular markers to be underexpressed. Multivariate analysis in patients with COPD identified membranous ß-Catenin as a differential biomarker, which displayed an underexpression, with an Odds Ratio (95% Confidence Interval) of 0.26 (0.07-1.01). A significant lowest expression of membranous ß-catenin was detected in NSCLC of the patients with COPD. Histol Histopathol 24, 417-423 (2009)
Key words: COPD, Immunohistochemistry, Molecular Biology, Non Small Cell Lung Cancer, Protein expression
DOI: 10.14670/HH-24.417