HISTOLOGY AND HISTOPATHOLOGY

Cellular and Molecular Biology

 

Expression pattern of squamous cell carcinoma antigen in oesophageal dysplasia and squamous cell carcinoma

A. Parenti1, A. Porzionato2, S. Pizzi1, V. Guzzardo1, G. Fassina3, V. Macchi2, V. Ninfo1 and R. De Caro2

1Section of Pathologic Anatomy, Department of Medical Diagnostic Sciences and Special Therapies, University of Padova, Italy, 2Section of Anatomy, Department of Human Anatomy and Physiology, University of Padova, Italy and 3Xeptagen, Pozzuoli (Na), Italy

Offprint requests to: Raffaele De Caro, MD., Section of Anatomy, Department of Human Anatomy and Physiology, University of Padova, Via A Gabelli 65, 35121 Padova, Italy. e-mail: rdecaro@unipd.it


Summary. The aim of the present study was to evaluate the tissue expression of squamous cell carcinoma antigen (SCCA) in oesophageal dysplasia and squamous cell carcinoma (SCC) with reference to its clinico-pathologic and prognostic significance. Immunohistochemistry using SCCA polyclonal antibody was performed on SCCs from 61 surgical oesophagectomies. Fifteen cases of low-grade dysplasia (LGD) and 37 non-coexistent high-grade dysplasia (HGD) were also sampled from these materials, together with sixteen chronic cases of oesophagitis. SCCA immunoreactivity was present in the maturative compartments of all normal epithelia and oesophagitis. LGDs showed no SCCA immunoreactivity in the dysplastic proliferative component but only in the superficial normal layers. In 94.6% of HGDs, no SCCA immunoreactivity was detected throughout the thickness of the epithelium. In SCCs, SCCA expression higher than 25% was found in 54% of cases. SCCA positivity showed an inverse correlation with histological grade, whereas no statistically significant correlation was found with TNM classifications, stage, or survival. SCCA is not expressed in early oesophageal carcinogenesis but, in SCC, it represents an indicator of histologic differentiation. In differentiated SCC, SCCA may represent a negative factor for cancer invasiveness, through inhibition of proteases. Histol Histopathol 22, 989-995 (2007)

Key words: Squamous cell carcinoma antigen, Oesophageal dysplasia, Immunohistochemistry

DOI: 10.14670/HH-22.989