The effect of the flavonoid diosmin, grape seed extract and red wine on the pulmonary metastatic B16F10 melanoma
C. Martínez1, V. Vicente1, J. Yáñez1, M. Alcaraz2, M.T. Castells3, M. Canteras4, O. Benavente-García4 and J. Castillo5
1Pathology Department. Faculty of Medicine, University of Murcia, Espinardo, Murcia, Spain, 2Radiology and Physical Medicine Department, Faculty of Medicine, University of Murcia, Espinardo, Murcia, Spain, 3Cell Biology Department, Faculty of Medicine, University of Murcia, Espinardo, Murcia, Spain, 4Biostatistics Department. Faculty of Medicine. University of Murcia, Espinardo, Murcia, Spain and 5Research and Development Department of Furfural Español S.A. Camino Viejo de Pliego s/n. Murcia. Spain
Offprint requests to: Vicente Vicente Ortega, Cátedra de Anatomía Patológica, Facultad de Medicina, Universidad de Murcia, Campus de Espinardo, 30100 Murcia, Spain. e-mail: vvortega@um.es
Summary. Objective: To study the effect of different phenolic compounds and red wine on pulmonary metastatic melanoma. Methods: Swiss mice were inoculated with 5x105 melanocytes B16F10 and given oral doses of diosmin, grape seed extract (GSE) and red wine. A macroscopic count was made of the metastatic nodules on the lung surface and a microscopic study by image analysis of five sections, calculating the implantation percentage and tumoral growth and invasion indices. Results: Macroscopically, the group treated with diosmin showed the greatest reduction (52%) in the number of metastatic nodules compared with the control group, which was treated with ethanol, while GSE and red wine caused decreases of 26.07 and 28.81%, respectively. Microscopically, there was a decrease in the implantation percentage after the administration of diosmin (79.4%) and red wine (20.19%), and an increase of 2.12% after the administration of GSE, all relative to the ethanol-treated control. As regards the growth index, diosmin produced a reduction of 67.44% and red wine a reduction of 20.62%, while GSE again produced an increase (25.33%). The reductions in the invasion index were 45.23, 31.65 and 17.57% with diosmin, GSE and red wine, respectively. Conclusions: Diosmin originated the greatest reduction in pulmonary metastases, both at the macroscopic and microscopic levels. Histol Histopathol 20, 1121-1129 (2005)
Key words: B16F10, Pulmonary metastasis, Procyanidins, Flavonoids, Diosmin
DOI: 10.14670/HH-20.1121